By Margaret V. Root Kustritz
Clinical canines and pussycat copy: Evidence-Based Answers provides fast, trustworthy solutions to the commonest questions in canines and tom cat reproductive and pediatric perform. Written utilizing an cutting edge question-and-answer structure, every one solution is designed for speedy reference, with the easiest references indexed for extra info as wanted. according to the author’s years of expertise answering questions about replica and pediatrics, Clinical Canine and Feline Reproduction permits the training veterinarian to swiftly locate and follow evidence-based solutions from the medical literature to their medical questions.
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Additional resources for Clinical Canine and Feline Reproduction: Evidence-Based Answers
Testicular ultrasound is a more accurate indicator of true testicular volume than is external measurement of testicular size with a pair of calipers. Supplemental reading Gouletsou PG, Galatos AD, Leontides LS. 2008. Comparison between ultrasonographic and caliper measurements of testicular volume in the dog. Anim Repro Sci 108:1–12. Johnston SD, Root Kustritz MV, Olson PN. 2001. Canine and Feline Theriogenology. , pp. 312–322. CANINE TECHNIQUES 9 What is the technique for prostatic fine-needle aspirate or biopsy?
Shave and surgically prep the pre-scrotal area as for a castration. Make a single pre-scrotal incision and advance one testis to the incision. Cut through the spermatic fascia and vaginal tunics to expose the testis within the tunica albuginea. To retrieve a biopsy sample, several techniques are described. One is to stab through the testicular capsule with a small scalpel blade. A portion of the testis will well through the capsular break (Fig. 11-1). Gently shave off this piece of testicular tissue.
Fill the pipette by capillary action. Squeeze the diluent container and insert the pipette, letting go of everything at once so the semen is sucked from the pipette into the diluent. Remove the pipette and turn it around, reseating the hub to form a dispenser. With either technique, spermatozoa are counted using a hemacytometer. Place the glass cover slip over the central area of the hemacytometer. Dispense diluted semen such that capillary action carries it across the central area. Fill each side of the hemacytometer separately.