By Rana S. Hoda
This booklet is meant as a pragmatic primer at the Pap try out. utilizing daring textual content, tables, and highlighted components, this e-book deals a common textual content on Pap attempt basics so readers may well locate particular info easily. The publication is an invaluable software for cytotechnologists and cytopathologists, in addition to these in education. Readers getting ready for numerous talent and licensing examinations also will locate necessary info all through.
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Additional resources for Fundamentals of Pap Test Cytology (Current Clinical Pathology)
These cells also line the endocervical glands present in the cervical stroma. Nabothian cysts form when the openings of these glands are plugged by mucus. The cells in the upper portion of the endocervical canal may be ciliated (tubal metaplasia). • Squamocolumnar junction: also known as the transformation zone (TZ) is the point where the stratified squamous epithelium of the ectocervix meets the endocervical glandular epithelium. The position of the TZ varies with age. In adolescents and young adults (and in certain pathological conditions), the endocervical epithelium is seen on the surface of the ectocervix (ectropion).
Spray fixatives contain isopropanol and polyethylene glycol (carbowax) that protects the cells from drying. The nozzle of the spray fixative should be about 12-inches away from the surface of the slide for optimal fixation of cells. The slides are then transported to the laboratory in cardboard containers. The carbowax should be removed by washing smears in 50% alcohol for 5–10 minutes before staining them. LBP are fixed immediately upon collection in the fixative sample collection media. Papanicolaou Stain • Papanicolaou stain the universal stain used for staining Pap test slides.
5 (H&E stain). Table 5 Decidual Cells vs Low-Grade Squamous Intra-Epithelial Lesions Features Cytoplasm Koilocytosis Nuclei Nucleolus History Decidual cells Abundant, pale and degenerative Absent Smooth contours Prominent, basophilic Helpful (pregnancy, postpartum) LSIL Mature Present with HPV “Raisinoid” with HPV Absent May not help Note: abundant pale cytoplasm and central round nucleus with prominent nucleoli differentiates decidual cells from LSIL. HPV, human papillomavirus; LSIL, low-grade squamous intra-epithelial lesion.