By Baochang Cai, Seng Poon Ong, Xunhong Liu
This e-book offers a finished and updated account of the HPLC know-how and fingerprints of commonly-used chinese language herbs. It comprises HPLC fingerprints of over forty conventional chinese language medicinal herbs and information in their resources, creation and quality controls. The ebook presents a pragmatic evaluate of the suggestions, suitable fabrics, and the actual calls for for utilizing HPLC in conventional chinese language medicinal herb functions. It additionally covers HPLC equipment used to research the herbs.
High-performance liquid chromatography (HPLC) is more and more utilized in the sector of conventional chinese language medication for acquiring fingerprints of chinese language medicinal herbs. merits reminiscent of excessive pace, excessive answer, excessive sensitivity, balance, accuracy, and automation make it one of many best strategies used for qc, standardization of planting, and new drug improvement in conventional chinese language medicine.
To date, no different books on HPLC fingerprints of conventional chinese language medicinal herbs were released in English. this can be the 1st publication in English dedicated to delivering readers with cutting-edge info on HPLC utilized within the research of conventional chinese language medicinal herbs.
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Additional info for High Performance Liquid Chromatography Fingerprinting Technology of the Commonly-Used Traditional Chinese Medicine Herbs
For the past few years, with the advancement of instruments and methods, the combination of HSCCC and mass spectrometry (HSCCC-MS) has successfully been applied in the separation, analysis and identification of chemical components in Chinese medicine. In the research of Chinese medicine quality control and analysis, especially in the field of fingerprint analysis, HSCCC has broad and favorable application prospects. indd 37 12/13/11 11:32:13 AM FA Reading 38 General Introduction Supercritical fluid chromatography Supercritical fluid chromatography utilizes a supercritical fluid as the mobile phase.
Indd 28 12/13/11 11:32:13 AM FA Reading Outline 29 and chemical oscillation technology. In actual applications, we can select one or more methods according to our needs. Photometry Photometry refers to the use of light of a certain wavelength to scan the sample and obtain the characteristic spectra and data. The main methods include ultraviolet (UV) spectrum, infrared (IR) spectrum, fluorescence spectrum (FS), nuclear magnetic resonance (NMR) and mass spectrometry (MS). In addition, there is atomic absorption spectrometry (AAS), inductively coupled plasma (ICP) and X-ray fluorescence (XRF).
After separation, a reproducible pyrolysis–gas chromatogram which reveals the fingerprint characteristics will be obtained from the recording instrument. GCRIS is based on the GC retention indices of the volatile components in Chinese medicine. From these, the retention indices of certain characteristic components are selected to make up the gas chromatographic retention index spectra, such that the characteristic fingerprint is more distinctive. GC-MS is widely applied and is able to provide “on-line” information about the chemical structure of the main components in the fingerprint.